MED22

MED22
Identifiers
Aliases	MED22, MED24, SURF5, surf-5, mediator complex subunit 22, SRB6
External IDs	OMIM: 185641 MGI: 98446 HomoloGene: 4913 GeneCards: MED22
Gene location (Human)
Chr.	Chromosome 9 (human)[1]
End	133,348,131 bp[1]
Gene location (Mouse)
Chr.	Chromosome 2 (mouse)[2]
End	26,910,677 bp[2]
RNA expression pattern
	More reference expression data
Gene ontology
Molecular function	• GO:0001104 transcription cofactor activity; • GO:0001948 protein binding;
Cellular component	• cytoplasm; • mediator complex; • cell nucleus;
Biological process	• regulation of transcription from RNA polymerase II promoter; • regulation of transcription, DNA-templated; • transcription, DNA-templated;
	Sources:Amigo / QuickGO
Orthologs
	6837
	20933
	ENSG00000281022; ENSG00000148297
	ENSMUSG00000015776
	Q15528; Q5T8T8
	Q62276
	NM_181491; NM_006752; NM_133640
	NM_001033908; NM_011513; NM_001378786; NM_001378787; NM_001378788
	NP_598395; NP_852468
	NP_001029080; NP_035643; NP_001365715; NP_001365716; NP_001365717
	Wikidata
View/Edit Human	View/Edit Mouse

Mediator of RNA polymerase II transcription subunit 22 is an enzyme that in humans is encoded by the MED22 gene.[5][6][7]

Function

This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene.[7]

Interactions

MED22 has been shown to interact with MED30.[8]

Model organisms

Model organisms have been used in the study of MED22 function. A conditional knockout mouse line called Med22^{tm1a(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute.[9] Male and female animals underwent a standardized phenotypic screen[10] to determine the effects of deletion.[11][12][13][14] Additional screens performed: - In-depth immunological phenotyping[15]

*Med22* knockout mouse phenotype
Characteristic	Phenotype
All data available at.[10][15]
Insulin	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Abnormal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Micronucleus test	Abnormal
Salmonella infection	Normal

References

ENSG00000148297 GRCh38: Ensembl release 89: ENSG00000281022, ENSG00000148297 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000015776 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Yon J, Jones T, Garson K, Sheer D, Fried M (Mar 1993). "The organization and conservation of the human Surfeit gene cluster and its localization telomeric to the c-abl and can proto-oncogenes at chromosome band 9q34.1". Human Molecular Genetics. 2 (3): 237–40. doi:10.1093/hmg/2.3.237. PMID 8499913.
Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell. 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
"Entrez Gene: SURF5 surfeit 5".
Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". The Journal of Biological Chemistry. 278 (17): 15123–7. doi:10.1074/jbc.C300054200. PMID 12584197.
Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88. doi:10.1111/j.1755-3768.2010.4142.x.
"International Mouse Phenotyping Consortium".
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
"Infection and Immunity Immunophenotyping (3i) Consortium".

De Falco S, Russo G, Angiolillo A, Pietropaolo C (Apr 1993). "Human L7a ribosomal protein: sequence, structural organization, and expression of a functional gene". Gene. 126 (2): 227–35. doi:10.1016/0378-1119(93)90371-9. PMID 8482538.
Garson K, Duhig T, Armes N, Colombo P, Fried M (Nov 1995). "Surf5: a gene in the tightly clustered mouse surfeit locus is highly conserved and transcribed divergently from the rpL7A (Surf3) gene". Genomics. 30 (2): 163–70. doi:10.1006/geno.1995.9889. PMID 8586415.
Garson K, Duhig T, Fried M (1997). "Tissue-specific processing of the Surf-5 and Surf-4 mRNAs". Gene Expression. 6 (4): 209–18. PMC 6148271. PMID 9196076.
Duhig T, Ruhrberg C, Mor O, Fried M (Aug 1998). "The human Surfeit locus". Genomics. 52 (1): 72–8. doi:10.1006/geno.1998.5372. PMID 9740673.
Angiolillo A, Russo G, Porcellini A, Smaldone S, D'Alessandro F, Pietropaolo C (Feb 2002). "The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts". Gene. 284 (1–2): 169–78. doi:10.1016/S0378-1119(02)00379-7. PMID 11891058.
Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". The Journal of Biological Chemistry. 278 (17): 15123–7. doi:10.1074/jbc.C300054200. PMID 12584197.
Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (Dec 2003). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex". The Journal of Biological Chemistry. 278 (50): 49671–4. doi:10.1074/jbc.C300444200. PMID 14576168.
Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (Feb 2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2". The Journal of Biological Chemistry. 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676.
Hillman RT, Green RE, Brenner SE (2005). "An unappreciated role for RNA surveillance". Genome Biology. 5 (2): R8. doi:10.1186/gb-2004-5-2-r8. PMC 395752. PMID 14759258.

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[refGRCh38Ensembl-1] ENSG00000148297 GRCh38: Ensembl release 89: ENSG00000281022, ENSG00000148297 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000015776 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8499913-5] Yon J, Jones T, Garson K, Sheer D, Fried M (Mar 1993). "The organization and conservation of the human Surfeit gene cluster and its localization telomeric to the c-abl and can proto-oncogenes at chromosome band 9q34.1". Human Molecular Genetics. 2 (3): 237–40. doi:10.1093/hmg/2.3.237. PMID 8499913.

[pmid15175163-6] Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Molecular Cell. 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.

[entrez-7] "Entrez Gene: SURF5 surfeit 5".

[pmid12584197-8] Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (Apr 2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3". The Journal of Biological Chemistry. 278 (17): 15123–7. doi:10.1074/jbc.C300054200. PMID 12584197.

[mgp_reference-9] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica. 88. doi:10.1111/j.1755-3768.2010.4142.x.

[IMPCsearch_ref-10] "International Mouse Phenotyping Consortium".

[pmid21677750-11] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-12] Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-13] Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.

[pmid23870131-14] White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.

[iii_ref-15] "Infection and Immunity Immunophenotyping (3i) Consortium".

MED22

Function

Interactions

Model organisms

References

Further reading