PRMT3

PRMT3
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	2FYT, 3SMQ, 4HSG, 4QQN, 4RYL
Identifiers
Aliases	PRMT3, HRMT1L3, protein arginine methyltransferase 3
External IDs	OMIM: 603190 MGI: 1919224 HomoloGene: 24255 GeneCards: PRMT3
Gene location (Human)
Chr.	Chromosome 11 (human)[1]
End	20,509,338 bp[1]
Gene location (Mouse)
Chr.	Chromosome 7 (mouse)[2]
End	49,858,265 bp[2]
RNA expression pattern
	More reference expression data
Gene ontology
Molecular function	• metal ion binding; • methyltransferase activity; • transferase activity; • GO:0001948 protein binding; • protein-arginine N-methyltransferase activity; • nucleic acid binding; • histone-arginine N-methyltransferase activity; • protein-arginine omega-N asymmetric methyltransferase activity; • ribosome binding;
Cellular component	• ribosome; • cytoplasm; • cytosol; • cell nucleus;
Biological process	• methylation; • negative regulation of protein ubiquitination; • peptidyl-arginine N-methylation; • protein methylation; • regulation of transcription, DNA-templated; • peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; • histone arginine methylation;
	Sources:Amigo / QuickGO
Orthologs
	10196
	71974
	ENSG00000185238
	ENSMUSG00000030505
	O60678
	Q922H1
	NM_001145166; NM_001145167; NM_005788
	NM_133740
	NP_001138638; NP_001138639; NP_005779
	NP_598501
	Wikidata
View/Edit Human	View/Edit Mouse

Protein arginine N-methyltransferase 3 is an enzyme that in humans is encoded by the PRMT3 gene.[5][6]

Model organisms

*Prmt3* knockout mouse phenotype
Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Normal
Fertility	Normal
General Observations	Abnormal
Body weight	Abnormal[7]
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Abnormal[8]
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Abnormal[9]
Radiography	Normal
Body temperature	Normal
Eye morphology	Abnormal[10]
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Abnormal
Micronucleus test	Normal
Heart weight	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Eye Histopathology	Normal
Salmonella infection	Normal[11]
Citrobacter infection	Normal[12]
All tests and analysis from[13][14]

Model organisms have been used in the study of PRMT3 function. A conditional knockout mouse line, called Prmt3^{tm1a(EUCOMM)Wtsi}[15][16] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[17][18][19]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[13][20] Twenty seven tests were carried out on mutant mice and seven significant abnormalities were observed.[13] Fewer than predicted homozygous mutant mice survived until weaning due to hydrocephaly. The remaining tests were carried out on both heterozygous and homozygous mutant adult mice. Male heterzygous mice had a decreased respiratory quotient. Homozygous females had decreased body weight, length and bone mineral density. Homozygous males had abnormal peripheral blood lymphocyte counts and homozygotes of both sex had eye abnormalities.[13]

Interactions

PRMT3 has been shown to interact with RPS2.[21]

References

GRCh38: Ensembl release 89: ENSG00000185238 - Ensembl, May 2017
GRCm38: Ensembl release 89: ENSMUSG00000030505 - Ensembl, May 2017
"Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
"Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
Tang J, Gary JD, Clarke S, Herschman HR (July 1998). "PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation". The Journal of Biological Chemistry. 273 (27): 16935–45. doi:10.1074/jbc.273.27.16935. PMID 9642256.
"Entrez Gene: PRMT3 protein arginine methyltransferase 3".
"Body weight data for Prmt3". Wellcome Trust Sanger Institute.
"Indirect calorimetry data for Prmt3". Wellcome Trust Sanger Institute.
"DEXA data for Prmt3". Wellcome Trust Sanger Institute.
"Eye morphology data for Prmt3". Wellcome Trust Sanger Institute.
"Salmonella infection data for Prmt3". Wellcome Trust Sanger Institute.
"Citrobacter infection data for Prmt3". Wellcome Trust Sanger Institute.
Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
Mouse Resources Portal, Wellcome Trust Sanger Institute.
"International Knockout Mouse Consortium".
"Mouse Genome Informatics".
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, et al. (June 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
van der Weyden L, White JK, Adams DJ, Logan DW (June 2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
Choi S, Jung CR, Kim JY, Im DS (September 2008). "PRMT3 inhibits ubiquitination of ribosomal protein S2 and together forms an active enzyme complex". Biochimica et Biophysica Acta (BBA) - General Subjects. 1780 (9): 1062–9. doi:10.1016/j.bbagen.2008.05.010. PMID 18573314.

External links

PRMT3 human gene location in the UCSC Genome Browser.
PRMT3 human gene details in the UCSC Genome Browser.

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[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000185238 - Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000030505 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9642256-5] Tang J, Gary JD, Clarke S, Herschman HR (July 1998). "PRMT 3, a type I protein arginine N-methyltransferase that differs from PRMT1 in its oligomerization, subcellular localization, substrate specificity, and regulation". The Journal of Biological Chemistry. 273 (27): 16935–45. doi:10.1074/jbc.273.27.16935. PMID 9642256.

[entrez-6] "Entrez Gene: PRMT3 protein arginine methyltransferase 3".

[Body_weight-7] "Body weight data for Prmt3". Wellcome Trust Sanger Institute.

[Indirect_calorimetry-8] "Indirect calorimetry data for Prmt3". Wellcome Trust Sanger Institute.

[DEXA-9] "DEXA data for Prmt3". Wellcome Trust Sanger Institute.

[Eye_morphology-10] "Eye morphology data for Prmt3". Wellcome Trust Sanger Institute.

[''Salmonella''_infection-11] "Salmonella infection data for Prmt3". Wellcome Trust Sanger Institute.

[''Citrobacter''_infection-12] "Citrobacter infection data for Prmt3". Wellcome Trust Sanger Institute.

[mgp_reference-13] Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.

[14] Mouse Resources Portal, Wellcome Trust Sanger Institute.

[allele_ref-15] "International Knockout Mouse Consortium".

[mgi_allele_ref-16] "Mouse Genome Informatics".

[pmid21677750-17] Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, et al. (June 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.

[mouse_library-18] Dolgin E (June 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.

[mouse_for_all_reasons-19] Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.

[pmid21722353-20] van der Weyden L, White JK, Adams DJ, Logan DW (June 2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

[pmid18573314-21] Choi S, Jung CR, Kim JY, Im DS (September 2008). "PRMT3 inhibits ubiquitination of ribosomal protein S2 and together forms an active enzyme complex". Biochimica et Biophysica Acta (BBA) - General Subjects. 1780 (9): 1062–9. doi:10.1016/j.bbagen.2008.05.010. PMID 18573314.

PRMT3

Model organisms

Interactions

References

Further reading

External links