Nudix hydrolase
Nudix hydrolases are a superfamily of hydrolytic enzymes capable of cleaving nucleoside diphosphates linked to x (any moiety), hence their name.[1][2][3] The reaction yields nucleoside monophosphate (NMP) plus X-P. Substrates hydrolysed by nudix enzymes comprise a wide range of organic pyrophosphates, including nucleoside di- and triphosphates, dinucleoside and diphosphoinositol polyphosphates, nucleotide sugars and RNA caps, with varying degrees of substrate specificity.[3] Enzymes of the Nudix superfamily are found in all types of organisms, including eukaryotes, bacteria and archaea.[3]
| NUDIX | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Structure of MT-ADPRase, a Nudix hydrolase from Mycobacterium tuberculosis | |||||||||
| Identifiers | |||||||||
| Symbol | NUDIX | ||||||||
| Pfam | PF00293 | ||||||||
| Pfam clan | CL0261 | ||||||||
| InterPro | IPR000086 | ||||||||
| |||||||||
| NUDIX-like | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Crystal structure of NADH pyrophosphatase (1790429) from Escherichia coli k12 at 2.20 a resolution | |||||||||
| Identifiers | |||||||||
| Symbol | NUDIX-like | ||||||||
| Pfam | PF09296 | ||||||||
| Pfam clan | CL0261 | ||||||||
| InterPro | IPR015375 | ||||||||
| SCOPe | 1vk6 / SUPFAM | ||||||||
| |||||||||
There are two components to the Nudix family: the so-called Nudix fold of a beta sheet with alpha helices on each side and the Nudix motif which contains catalytic and metal-binding amino acids. The Nudix motif is GXXXXXEXXXXXXXREUXEEXGU where U is isoleucine, leucine or valine, and X is any amino acid. This forms a short helix which (usually) contains the catalytic amino acids. Nudix hydrolases include Dcp2 of the decapping complex, ADP-ribose diphosphatase, MutT, ADPRase, Ap4A hydrolases, RppH, and many others.[4]
References
- Bessman MJ, Frick DN, O'Handley SF (October 1996). "The MutT proteins or "Nudix" hydrolases, a family of versatile, widely distributed, "housecleaning" enzymes". J. Biol. Chem. 271 (41): 25059–62. doi:10.1074/jbc.271.41.25059. PMID 8810257.
- Mildvan AS, Xia Z, Azurmendi HF, et al. (January 2005). "Structures and mechanisms of Nudix hydrolases". Arch. Biochem. Biophys. 433 (1): 129–43. doi:10.1016/j.abb.2004.08.017. PMID 15581572.
- McLennan AG (January 2006). "The Nudix hydrolase superfamily". Cell. Mol. Life Sci. 63 (2): 123–43. doi:10.1007/s00018-005-5386-7. PMID 16378245.
- Mildvan, A.S.; Xia, Z.; Azurmendi, H.F.; Saraswat, V.; Legler, P.M.; Massiah, M.A.; Gabelli, S.B.; Bianchet, M.A.; et al. (2005). "Structures and mechanisms of Nudix hydrolases". Archives of Biochemistry and Biophysics. 433 (1): 129–143. doi:10.1016/j.abb.2004.08.017. PMID 15581572.