N2a cell

N2a cells (also known as Neuro2a cells) are a fast-growing mouse neuroblastoma cell line.[1]

Differentiation Properties

Originating from a mouse, the N2a cell line has a neuronal and amoeboid stem cell morphology, allowing it to differentiate in response to environmental factors. The differentiated cells have many properties of neurons, including neurofilaments. The cells, due to passaging since initial collection, can exhibit responses to toxins that differ from those of neuronal cells in a live organism.[2] Synthesizing large amounts of microtubules, N2a cells are susceptible to viruses (such as herpes simplex and poliovirus) that can alter cell morphology and physiology.

Research Applications

N2a cells have been used to study neurite outgrowth,[1] neurotoxicity,[2] Alzheimer's disease,[3], asymmetric division of mammalian cell lines[4], adenoviral transduction, and the diagnosing of rabies.

References
  1. Salto R, Vílchez JD, Girón MD, Cabrera E, Campos N, Manzano M, Rueda R, López-Pedrosa JM (August 2015). "β-Hydroxy-β-Methylbutyrate (HMB) Promotes Neurite Outgrowth in Neuro2a Cells". PLOS One. 10 (8): e0135614. doi:10.1371/journal.pone.0135614. PMC 4534402. PMID 26267903.
  2. LePage KT, Dickey RW, Gerwick WH, Jester EL, Murray TF (2005). "On the use of neuro-2a neuroblastoma cells versus intact neurons in primary culture for neurotoxicity studies". Critical Reviews in Neurobiology. 17 (1): 27–50. doi:10.1615/critrevneurobiol.v17.i1.20. PMID 16307526.
  3. Provost P (2010). "Interpretation and applicability of microRNA data to the context of Alzheimer's and age-related diseases". Aging. 2 (3): 166–169. doi:10.18632/aging.100131. PMC 871245. PMID 20375468.
  4. Ogrodnik M, Salmonowicz H, Brown R, Turkowska J, Sredniawa W, Pattabiraman S, Amen T, Abraham AC, Eichler N, Lyakhovetsky R, Kaganovich D (2014). "Dynamic JUNQ inclusion bodies are asymmetrically inherited in mammalian cell lines through the asymmetric partitioning of vimentin". Proceedings of the National Academy of Sciences of the United States of America. 111 (22): 8049–54. doi:10.1073/pnas.1324035111. PMC 4050583. PMID 24843142.


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