MAGESTIC

Multiplexed Accurate Genome Editing with Short, Trackable, Integrated Cellular barcodes (MAGESTIC) is a platform that builds on the CRISPR/Cas technique. It further improves CRISPR/Cas by making the gene-editing process more precise. It also increases cell survival during the editing process up to sevenfold.[1][2][3]

The platform was made by the Joint Initiative for Metrology in Biology (JIMB)[4] which is a coalition of Stanford University and the National Institute of Standards and Technology.

Overview

Gene editing is used for a variety of tasks including the modifying of crops, the modifying of bacteria, and the modifying of disease-causing genetic mutations in patients. For industrial and agricultural uses, CRISPR/Cas is more than sufficient, but for medicinal use, more precision is preferred. This is where MAGESTIC comes in. MAGESTIC achieves greater precision by using array-synthesized guide–donor oligos for the plasmid-based high-throughput editing and it also features a genomic barcode integration to prevent plasmid barcode loss (and also to enable robust phenotyping).

In essence, it allows to program the CRISPR machinery to cut at desired locations throughout the genome, and then to direct the cells to introduce designed edits at the DNA cut sites. As such, it is a very important tool for making gene therapies.

It is downloadable via k-roy's download page, also mentioned at the full paper of the MAGESTIC article from Nature biotechnology.

References
  1. From Clipping Scissors to Word Processor
  2. Roy, KR; Smith, JD; Vonesch, SC; Lin, G; Tu, CS; Lederer, AR; Chu, A; Suresh, S; Nguyen, M; Horecka, J; Tripathi, A; Burnett, WT; Morgan, MA; Schulz, J; Orsley, KM; Wei, W; Aiyar, RS; Davis, RW; Bankaitis, VA; Haber, JE; Salit, ML; St Onge, RP; Steinmetz, LM (2018). "Multiplexed precision genome editing with trackable genomic barcodes in yeast". Nat Biotechnol. 36: 512–520. doi:10.1038/nbt.4137. PMC 5990450. PMID 29734294.
  3. Multiplexed precision genome editing with trackable genomic barcodes in yeast
  4. JIMB website
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