DNA construct

A DNA construct is an artificially constructed segment of nucleic acid that is to be transplanted into a target tissue or cell.[1] Plasmid DNA constructs often consist of a promoter sequence, followed a by a desired gene, and ended in a transcription termination or polyadenylation signal sequence.[2] A DNA construct may contain a DNA insert, which contains the gene sequence encoding a protein of interest, that has been subcloned into a vector, which contains bacterial resistance genes for growth in bacteria, and promoters for expression in the organism. A DNA construct may express wildtype protein, prevent the expression of certain genes by expressing competitors or inhibitors, or express mutant proteins, such as deletion mutations or missense mutations. A DNA construct is often used in molecular biology to analyze macromolecules such as proteins or RNA in more detail.