Chu 13

CHU 13 medium is a culture medium used in microbiology for the growth of certain algal species, first published by S.P. Chu in 1942.[1] It is used as growth medium for the biofuel candidate alga Botryococcus braunii.[2]

Botryococcus braunii

CHU 13 includes essential minerals and trace elements that are required by algae for growth, but does not include a carbon source and so is only appropriate for growth of phototrophs. It can be prepared as either a liquid medium or as an agar medium.

Modified CHU13 Medium[3]
Compoundmg/L
KNO3400
K2HPO480
CaCl2 dihydrate107
MgSO4 heptahydrate200
Ferric citrate20
Citric acid100
CoCl20.02
H3BO35.72
MnCl2 tetrahydrate3.62
ZnSO4 heptahydrate0.44
CuSO4 pentahydrate0.16
Na2MoO40.084
0.072 N H2SO41 drop

The remaining volume is pure, de-ionized water.

Because it is difficult to weigh out some of the trace minerals, it is advisable to create a mixture of all components at a large concentration, such as a thousand times these measures, and then mix with the appropriate amount of (pure, de-ionized) water. Correct pH to 7.5, and then autoclave.

References
  1. S.P. Chu (Aug., 1942) The Journal of Ecology, Vol. 30, No. 2, pp. 284-325. https://www.cpp.edu/~biodiesel/documents/reference/Chu42AlgaIsolGrowth.pdf
  2. C. Largeau, E. Casadevall, C. Berkaloff and P. Dhamelincout (1980) Phytochemistry, 19, 1943.
  3. K. Yamaguchi; et al. (1987). "Lipid composition of a green alga, Botryococcus braunii". Agric. Biol. Chem. 51 (2): 493–498. doi:10.1271/bbb1961.51.493.
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