50S ribosomal protein L25

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Structures	Swiss-model
Domains	InterPro

50S ribosomal subunit protein L25
	Crystal structure of e. coli ribosomal protein l25 complexed with a 5S rRNA fragment based on PDB: 1DFU
Identifiers
Organism	Escherichia coli (strain K-12 substrain MG1655)
Symbol	rplY
Entrez	945618
PDB	1DFU
UniProt	P68919
Other data
Chromosome	genome: 2.28 - 2.28 Mb
Search for
Structures	Swiss-model
Domains	InterPro

50S ribosomal protein L25 is a protein that in Escherichia coli is encoded by the rplY gene.

Function

This ribosomal protein is a component of the 50S subunit. The protein binds 5S rRNA to form a stable complex. In turn 5S rRNA binds specifically to three proteins, L25, L18 and L5, forming a separate domain of the bacterial ribosome.[1] Protein L25 of E. coli is not essential for survival of the cells.[2][3]

Interactions

Ribosomal protein L25 has been shown to interact with:

50S ribosomal protein L16[4][5][6]
5S ribosomal RNA[4][5][6]

References

Chen-Schmeisser U, Garrett RA (March 1977). "A new method for the isolation of a 5 S RNA complex with proteins L5, L18 and L25 from Escherichia coli ribosomes". FEBS Letters. 74 (2): 287–91. doi:10.1016/0014-5793(77)80866-1. PMID 321249.
Korepanov AP, Gongadze GM, Garber MB, Court DL, Bubunenko MG (March 2007). "Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli". Journal of Molecular Biology. 366 (4): 1199–208. doi:10.1016/j.jmb.2006.11.097. PMC 1939977. PMID 17198710.
Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection". Molecular Systems Biology. 2: 2006.0008. doi:10.1038/msb4100050. PMC 1681482. PMID 16738554.
Harms J, Schluenzen F, Zarivach R, Bashan A, Gat S, Agmon I, Bartels H, Franceschi F, Yonath A (2001). "High resolution structure of the large ribosomal subunit from a mesophilic eubacterium". Cell. 107 (5): 679–88. doi:10.1016/S0092-8674(01)00546-3. PMID 11733066.
Selmer M, Dunham CM, Murphy FV, Weixlbaumer A, Petry S, Kelley AC, Weir JR, Ramakrishnan V (September 2006). "Structure of the 70S ribosome complexed with mRNA and tRNA". Science. 313 (5795): 1935–42. doi:10.1126/science.1131127. PMID 16959973.
Schuwirth BS, Borovinskaya MA, Hau CW, Zhang W, Vila-Sanjurjo A, Holton JM, Cate JH (November 2005). "Structures of the bacterial ribosome at 3.5 A resolution". Science. 310 (5749): 827–34. doi:10.1126/science.1117230. PMID 16272117.

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[1] Chen-Schmeisser U, Garrett RA (March 1977). "A new method for the isolation of a 5 S RNA complex with proteins L5, L18 and L25 from Escherichia coli ribosomes". FEBS Letters. 74 (2): 287–91. doi:10.1016/0014-5793(77)80866-1. PMID 321249.

[2] Korepanov AP, Gongadze GM, Garber MB, Court DL, Bubunenko MG (March 2007). "Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli". Journal of Molecular Biology. 366 (4): 1199–208. doi:10.1016/j.jmb.2006.11.097. PMC 1939977. PMID 17198710.

[3] Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection". Molecular Systems Biology. 2: 2006.0008. doi:10.1038/msb4100050. PMC 1681482. PMID 16738554.

[Harms_2001-4] Harms J, Schluenzen F, Zarivach R, Bashan A, Gat S, Agmon I, Bartels H, Franceschi F, Yonath A (2001). "High resolution structure of the large ribosomal subunit from a mesophilic eubacterium". Cell. 107 (5): 679–88. doi:10.1016/S0092-8674(01)00546-3. PMID 11733066.

[Selmer_2006-5] Selmer M, Dunham CM, Murphy FV, Weixlbaumer A, Petry S, Kelley AC, Weir JR, Ramakrishnan V (September 2006). "Structure of the 70S ribosome complexed with mRNA and tRNA". Science. 313 (5795): 1935–42. doi:10.1126/science.1131127. PMID 16959973.

[Schuwirth_2005-6] Schuwirth BS, Borovinskaya MA, Hau CW, Zhang W, Vila-Sanjurjo A, Holton JM, Cate JH (November 2005). "Structures of the bacterial ribosome at 3.5 A resolution". Science. 310 (5749): 827–34. doi:10.1126/science.1117230. PMID 16272117.