Hexadimethrine bromide

Hexadimethrine bromide
Names
	IUPAC name 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide
	Other names Polybrene
Identifiers
CAS Number	28728-55-4 ;
ChemSpider	none;
ECHA InfoCard	100.209.698
Properties
Chemical formula	(C13H30Br2N2)n, linear form
Molar mass	variable
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
	Infobox references

Hexadimethrine bromide (commercial brand name Polybrene) is a cationic polymer used to increase the efficiency of transduction of certain cells with retrovirus in cell culture.^[1] Hexadimethrine bromide acts by neutralizing the charge repulsion between virions and sialic acid on the cell surface.^[2] Use of Polybrene can improve transduction efficiency 100-1000 fold^[3] although it can be toxic to some cell types. Polybrene in combination with DMSO shock is used to transfect some cell types such as NIH-3T3 and CHO.^[3] It has other uses, including a role in protein sequencing.^[4]

Historically, hexadimethrine bromide was sometimes used to reverse heparin anticoagulation during open-heart surgery. Its use has been supplanted by protamine sulfate.

Polybrene is also used in enzyme kinetic assays in order to reduce spontaneous activation of zymogens that are prone to auto activation.

References

↑ Howard e. Davis; Jeffery R. Morgan; Martin L. Yarmush (2002). "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on the target cell membranes". Biophysical Chemistry. 97 (2): 159–172. doi:10.1016/S0301-4622(02)00057-1.
↑ Howard E. Davis; Matthew Rosinski; Jeffrey R. Morgan; Martin L. Yarmush; et al. (2004). "Charged Polymers Modulate Retrovirus Transduction via Membrane Charge Neutralization and Virus Aggregation". Biophysical Journal. 86 (2): 1234–42. doi:10.1016/S0006-3495(04)74197-1. PMC 1303915. PMID 14747357.
1 2 "Polybrene Infection / Transfection Reagent | TR-1003-G". www.emdmillipore.com. Retrieved 2016-12-02.
↑ Hunkapiller, M. W.; Hood, L. E. (1978-05-30). "Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (polybrene), and high pressure liquid chromatography". Biochemistry. 17 (11): 2124–2133. ISSN 0006-2960. PMID 667015.

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[1] Howard e. Davis; Jeffery R. Morgan; Martin L. Yarmush (2002). "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on the target cell membranes". Biophysical Chemistry. 97 (2): 159–172. doi:10.1016/S0301-4622(02)00057-1.

[2] Howard E. Davis; Matthew Rosinski; Jeffrey R. Morgan; Martin L. Yarmush; et al. (2004). "Charged Polymers Modulate Retrovirus Transduction via Membrane Charge Neutralization and Virus Aggregation". Biophysical Journal. 86 (2): 1234–42. doi:10.1016/S0006-3495(04)74197-1. PMC 1303915. PMID 14747357.

[:0-3] 1 2 "Polybrene Infection / Transfection Reagent | TR-1003-G". www.emdmillipore.com. Retrieved 2016-12-02.

[4] Hunkapiller, M. W.; Hood, L. E. (1978-05-30). "Direct microsequence analysis of polypeptides using an improved sequenator, a nonprotein carrier (polybrene), and high pressure liquid chromatography". Biochemistry. 17 (11): 2124–2133. ISSN 0006-2960. PMID 667015.