Acid fuchsin

Acid fuchsin
Names
	IUPAC name Disodium 2-amino-5-[(Z)-(4-amino-3-sulfonatophenyl)(4-iminio-3-sulfonato-2,5-cyclohexadien-1-ylidene)methyl]-3-methylbenzenesulfonate
Identifiers
CAS Number	3244-88-0 ;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:87052 ;
ChemSpider	16736101 ;
ECHA InfoCard	100.019.833
EC Number	221-816-5
PubChem CID	5464362;
	InChI InChI=1S/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2+1/p-2/b19-11-,21-14?;; Key: RZUBARUFLYGOGC-MTHOTQAESA-L ; InChI=1/C20H19N3O9S3.2Na/c1-10-6-13(9-18(20(10)23)35(30,31)32)19(11-2-4-14(21)16(7-11)33(24,25)26)12-3-5-15(22)17(8-12)34(27,28)29;;/h2-9,21H,22-23H2,1H3,(H,24,25,26)(H,27,28,29)(H,30,31,32);;/q;2+1/p-2/b19-11-,21-14?;; Key: RZUBARUFLYGOGC-LOQRZINWBG;
	SMILES CC1=CC(=CC(=C1N)S(=O)(=O)[O-])/C(=C\2/C=CC(=[NH2+])C(=C2)S(=O)(=O)[O-])/C3=CC(=C(C=C3)N)S(=O)(=O)[O-].[Na+].[Na+];
Properties
Chemical formula	C20H17N3Na2O9S3
Molar mass	585.53 g·mol−1
Hazards
GHS pictograms
GHS signal word	Warning
GHS hazard statements	H315, H319, H335
GHS precautionary statements	P261, P264, P271, P280, P302+352, P304+340, P305+351+338, P312, P321, P332+313, P337+313, P362, P403+233, P405, P501
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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	Infobox references

Acid fuchsin or fuchsine acid is an acidic magenta dye with chemical formula C₂₀H₁₇N₃Na₂O₉S₃. It has wide use in histology. It is one of the dyes used in Masson's trichrome stain.^[1] This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with light green SF yellowish or methyl blue. It can also be used to identify growing bacteria.^[2]

Formula:

1 g of acid fuchsin
1 mL of glacial acetic acid (HOAc)
100 mL of distilled water

References

↑ Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. ISBN 971-11-0853-4
↑ Holman, W. L (1914). "The use of Decolorized Acid Fuchsin as an Acid Indicator in Carbohydrate Fermentation Tests with some Remarks on Acid Production by Bacteria". Journal of Infectious Diseases. 15: 227–233. doi:10.1093/infdis/15.1.227.

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[1] Jocelyn H. Bruce-Gregorios, M.D.: Histopathologic Techniques, JMC Press Inc., Quezon City, Philippines, 1974. ISBN 971-11-0853-4

[2] Holman, W. L (1914). "The use of Decolorized Acid Fuchsin as an Acid Indicator in Carbohydrate Fermentation Tests with some Remarks on Acid Production by Bacteria". Journal of Infectious Diseases. 15: 227–233. doi:10.1093/infdis/15.1.227.

Acid fuchsin

See also

References