Biker Mice from Mars

Protagonists

"The Bros.", Throttle, Vinnie, Modo

• Throttle (voiced by Rob Paulsen)^[5] - The tan-furred leader-figure of the trio with a quiff and ponytail. He's the most logical, level-headed, rational and calculating of the three. Throttle lost his eyes in an incident on Mars that also caused the losses of Modo's right arm and the right side of Vinnie's face, and was given malfunctioning bionic eyes in captivity: as a result, he wears green sunglasses with field spec capabilities, and on his right hand he wears a powered glove which increases the strength of his blows with that fist. He also carries a holstered laser pistol and sports a leather biker vest, making him the only one of the trio to carry a weapon or wear clothes instead of armor. His first symbolic quote in the series was, "In this wild and woolly universe, there are three things you can count on: your brains, your bros and your bike!"
• Modo (voiced by Dorian Harewood) - The gray-furred gentle giant of the trio who sports an eye patch, suggesting that his eye may have been damaged during Karbunkle's experiments. He lost his right arm in the same incident that burned off the right side of Vinnie's face and destroyed Throttle's eyes. His robotic arm replacement has a built-in laser cannon and great strength, and he wears powered shoulder pads in addition to it. When angry, his right eye flashes red. He is prone to fits of rage when he or his bros are referred to as 'rats'; his first signature quote actually was "RAT?!! My mama didn't raise no stinkin' rat! UNDERSTAND?!!". Modo is by far the strongest of the three mice, but despite outwardly looking the most threatening and aggressive, he is the most empathetic and sensitive of the group, frequently referencing what his grayfurred mama used to say. The big guy doesn't want any harm to befall Charley, and as such, she 'mothers' him in return. His bike is the only one out of the three with a definite name ("Lil' Hoss"), and the one with the most demonstrated AI capability.
• Vinnie (voiced by Ian Ziering) - The white-furred thrill-seeker and self-proclaimed lady-killer among the trio, a classic example of an egomaniac (with an inferiority complex, especially during his early days as a Freedom Fighter). The right side of his face was burned off in the same incident where Throttle lost his eyes and Modo lost his right arm, thus he wears a flexible faceplate. Vinnie rides a red sport bike as opposed to the other's choppers and is the most active of the three, often volunteering for the most dangerous tasks, enjoying the rush and subsequent glory. He wears a green crossbelt on his chest and wields expandable flares. His bike seems to have the greatest arsenal of weaponry between the three, and he is the best overall biker of the trio. He has a recurring catchphrase, "What a rush!", and has a signature laugh of triumph. Vinnie is definitely the wildest, most fun-loving and egotistical of the group; all three are adrenaline junkies to the extreme, but even Throttle and Modo can't understand how he's still alive, considering the risks he takes. Despite his "hyper-masculinity", Vinnie is actually a very loving and caring individual. He flirts endlessly with Charley, who constantly rebuffs him, but anytime she does reciprocate, Vinnie can only blush in extreme shyness.
• Charlene "Charley" Davidson (voiced by Leeza Miller-McGee) - The Mice are always backed up by Charley, a auburn haired, green-eyed human mechanic, who owns the Last Chance Garage in Chicago. She is a headstrong woman and is always ready to go into battle, though the Biker Mice try to keep her out of dangerous situations, never because they think she's incapable or weak, but because they couldn't bear anything bad happening to her. Aside from being the target of Vinnie's affection (which she playfully blows off), she is also responsible for repairs and upgrades to the Mice's bikes, is a high-tech genius in her own right, and is as skilled a biker as the Mice. Her name is an obvious pun on Harley Davidson.
• Rimfire (voiced by Brian Austin Green) - Modo's nephew who is overprotected by his peers on Mars because of his age, despite the fact that he, too, is clearly capable of what it takes to be a worthy Freedom Fighter. He first appeared in the episode "Back to Mars, Part 2" when the three Mice were escaping prison. He appears again in the episode Stalkers, where he also crashed into the scoreboard at Quigley Field while escaping from intergalactic rogue bounty hunters (who view the Biker Mice as the big championship stock) that eventually request Limburger's permission, and he played a crucial role in their defeat. He also appears in the 3-part episode Once Upon a Time on Mars, where the viewers see for the first time how his age led to distrust from some of his peers.
• Carbine (voiced by Leah Remini) - General Carbine is the de facto leader of the Freedom Fighters, a former Army officer who defected during the Plutarkian-Mars war. She and Throttle are lovers, but their relationship suffers complications owing to her position among the Freedom Fighters.
• Stoker (voiced by Peter Strauss) - Stoker is the founding father of the Freedom Fighter movement on Mars, and as seen in Once Upon a Time on Mars, revealed that Mars was sold, beginning to have doubts over the fight; eventually he continues fighting as part of the rebellion. He plays a more crucial role in the 2006 revival, where he fights to protect his invention from the invading Catatonian Empire.
• Harley (voiced by Kath Soucie) - A mechanic/nurse among the Freedom Fighters. Was involved in a love triangle between Vinnie and Stoker, until Vinnie's injuries moved her enough to make him the faceplate and crossbelts he wears. Hardly had they come to an understanding, she was abducted towards the end of the Freedom Fighters' insurrection. She returns as an antagonist in Biker Mice from Mars (2006).

Antagonists

• Lawrence Limburger (voiced by W. Morgan Sheppard) - The main antagonist of the series and arch enemy of the Biker Mice. Lawrence Limburger is a bloated alien from Plutark and has an odd fascination for Earth's criminal society of the 1930s. He is the head of Limburger Industries, the biggest industrial company in Chicago. Wearing a purple business suit and a rubber mask in order to look human at most times, he attempts to mine it and other parts of Earth of various resources including random earth, dirt, rock, snow, metal and oil, which he plans to send back to his own dying planet. He is highly resourceful and cunning, but always failing due to the Biker Mice's interventions. His headquarters is a very tall tower called Limburger Plaza. Unfortunately for him, his plans are foiled and his tower is either destroyed, torn down, launched into space or even disappears completely by the Biker Mice at the climax of almost every single episode, a few episodes lampshades this by showing his tower apparently fine at the end only to collapse anyway when hit by something else usually caused by the events. Limburger reappears in the 2006 sequel series, initially as a bootlicker for the Pit Boss but later as a co-conspirator alongside the Catatonians.
• Dr. Benjamin Boris Zachary Karbunkle (voiced by Susan Silo) - A thin, sly mad scientist of species unknown. Karbunkle used to work for Limburger's superior Dominic T. Stilton until Limburger bribed him to cross over. Karbunkle's main task is to think of machines and robots to either battle the Biker Mice, gather resources from Earth, or look up the villain of the week with his dimensional transporter. What is common knowledge is that during the War of Liberation on Mars he gave Modo his bionic arm (though in the pilot he pretended to be unfamiliar with it, calling it an interesting invention), Stoker his bionic tail and Throttle his malfunctioning bionic eyes. Karbunkle was also the one who built the Mind Bender Beam, successfully used on Stoker to brainwash the Freedom Fighter leader into obeying the Plutarkians. Karbunkle is a sycophant, often seen admiring Limburger and praising him; however, Karbunkle's top priority is Karbunkle. He is often seen torturing Fred the Mutant, cares little about his co-worker Greasepit and has traded Plutarkian employers many times. Karbunkle is very sadistic and is known to enjoy torturing people, wears a white lab coat, high heeled boots, has a green pair of goggles which he never takes off and once in a while, his red underwear is revealed. Also reappears in the 2006 series as well, and suffers the same fate as Limburger.
• Greasepit (voiced by Brad Garrett) - The stereotypical cartoonish bungling bad guy sidekick usually in charge of whatever project Lawrence Limburger is running at the time. He fails in his endeavors most of the time, which Limburger soon learns to factor into his planning. Greasepit rides a motorized tricycle into battle against the Biker Mice at the head of his goons and wears a pair of gardening trousers like a mechanic's. As his name suggests, Greasepit constantly oozes oil, and he is consistently clumsy, often falling over or dropping critical pieces of Limburger's schemes. Greasepit is the first villain Limburger ever hired though, his advert was "spiced up" to make him seem more capable than the bungling goof he is by his agent (who is possible also his mother). Briefly reappeared in the 2006 series as a henchman for new series villain Ronaldo Rump during the end of the story arc.
• Fred the Mutant (voiced by Rob Paulsen) - Fred has a happy lot in life; he thrills at the thought of receiving pain and that is his one purpose as well, being the subject of many of Karbunkle's testings as well as physical abuse by Limburger. He is a midget with clothing similar to portrayals of Quasimodo, is bald and has three pink eyes with black irises, a bushy tail, and a tentacle instead of a right arm.
• Lord Camembert (voiced by Jeff Bennett) - Limburger's higher-up, whom Limburger finds disgusting especially when he makes him do the embarrassing Plutarkian greeting. In some other cases, he will appear with the entire Plutarkian council with Limburger in a panic over what task is current. He often appears on the vidcom or in person to Limburger and berates him for his failure, often at a loud voice. As High Chairman of Plutark Camembert holds highest power of the planet for four-year term at once.
• Pit Boss (voiced by Stu Rosen) - The Pit Boss is the burly ruler of the Pits outside of town. His men, referred to as his "Pit Crew" will come up and rob various places, often taken hostages as well. The Pit Boss has an electric whip that he uses to keep his slaves in line.
• Napoleon Brie (voiced by Luke Perry) - Limburger's chief Plutarkian rival from Detroit, except that Brie usually seems to be rather successful. He and his "Number one", a gun for hire with a big red beard and sunglasses, dominate Detroit. Despite this, Brie's attempts at taking out the Biker Mice turn out just as futile as Limburger's. Brie's efforts are not helped by his undermining Limburger at the same time. Small of stature and the owner of a large variety of facial masks although he only ever wears the one with a crazy eye and speaks with an accent very similar to Elmer Fudd.
• Mace (voiced by Jeff Bennett) - Mace is a Nomad rat who appears to be disguise as a mouse and into intervening with the mice and the plutarkian war in secret and sometimes coming into conflict with Vinnie, but he escaped by grabbing Harley and his fate is unknown.

Super villains

In most episodes, Limburger orders Karbunkle to use the transporter to bring into Chicago one of the very powerful super villains. Generally each of them have one special ability which is useful for Limburger's plan in hand. Most of them seem to use an asteroid station called Black Rock as their main hideout when they're not summoned by those willing to pay for their services. On one notable occasion in the episode "A Scent, a Memory, a Far Distant Cheese", Limburger and Karbunkle accidentally got sucked into the transporter and appear in a nightclub where all the villains confront him for not paying them as they asked for.